Fig. 1.

Overview of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) differentiation. A Schematic overview of the differentiation protocol utilised in this study (upper), and the process of long-term continuous culture on glass coverslips (lower). Early (young) hiPSC-CM underwent experimentation between 30 and 46 days after differentiation whilst late (old) hiPSC-CM were measured between day 47 to 80. B Immunofluorescent staining of hiPSC-CM at d29. C Flow cytometry analysis of hiPSC-CM at d29. D Longitudinal section area of early and late hiPSC-CM (left), corresponding cell capacitance (middle) and T-tubule density (right), estimated through a ratio of capacitance to longitudinal section area of each cell. E Representative photomicrographs of early (left) and late (right) hiPSC-CM. Scale bar represents 10 µm. Data are mean ± SEM. Symbols represent separate differentiations. n/N = number of hiPSC-CM/differentiation