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. 2023 Apr 5;13:5597. doi: 10.1038/s41598-023-31720-7

Table 1.

Antibodies/Reagent used for immunofluorescence staining.

1° Antibody/reagent Target Company Dilution
Neurofilament H (SMI-31) Axonal marker Biolegend 1:500
β3-tubulin (TUBB3) Neuronal marker Biolegend 1:2000
Stathmin-2 (STMN2) Early regeneration marker Novus 1:2000
GAP-43 Regeneration Marker Sigma-Aldrich 1:1000
Phalloidin 647 conjugate F-actin Invitrogen 1:400
Islet 1 & 2 (ISL 1/2) Motor neuron marker Developmental Studies Hybridoma Bank 1:50