Skip to main content
. 2023 Mar 23;14:1139329. doi: 10.3389/fimmu.2023.1139329

Figure 3.

Figure 3

Anti-Vi IgM induced by Vi vaccines is sufficient to impair infection. (A) Colony forming units (CFU) in the spleen of IgG1-/- mice immunized i.p with 2 µg Vi-PS, Vi-TT or PBS, then infected for 24 hours with 1x105 CFU Vi+ S. Typhimurium TH177 14 days later. Representative of 2 experiments. (B) Serum anti-Vi IgM titres of WT and AID-/- mice after the same challenge schedule were assessed by ELISA after absorption against BSA. (C) Spleen CFU counts of both C57B/6 (WT) and AID-/- mice. (D) Serum bactericidal activity of WT and AID-/- sera was measured against TH177, represented as mean change from the starting LogCFU/mL bacterial dose, normalised to the negative control values at each timepoint. Complete human serum (CHS) was used as a positive control. Error bars represent SEM. Representative of 2 experiments with n = 2-4 mice/group. *p<0.05, **p<0.01, and ns = non-significant by Mann-Whitney U test between individual groups (two-tailed).