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. 2023 Mar 23;14:1139329. doi: 10.3389/fimmu.2023.1139329

Figure 5.

Figure 5

Antibody responses to Vi-PS and TCVs over time. C57Bl/6 mice were immunized i.p with PBS, 4 µg Vi-PS on day 0, or 2 µg of Vi-CRM197 or Vi-TT on both day 0 and 35. Serum was collected day 185 (6 months post-primary immunization). Sera were assessed by ELISA for (A) anti-Vi IgM and IgG and (B) isotypes IgG1, IgG2b or IgG3. (C) Bone marrow anti-Vi IgG, IgG1 and IgG3 antibody secreting cells (ASCs) at the 6-month timepoint were enumerated by ELISPOT. (D) C57Bl/6 mice were immunized i.p with 4 µg Vi-PS or PBS on day 0, or 2 µg Vi-TT at both day 0 and 35. Serum was collected day at day 40, 110, 155 and 202 and anti-Vi IgM and IgG determined by ELISA. Data pooled from 2 experiments with n = 5-6 mice/group. *p≤0.05, **p≤0.01, ***p≤0.005, ****p≤0.001 and ns = non-significant by Mann-Whitney U test between individual groups (two-tailed).