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| Expected concentration (ng ml−1) | Spiked before extraction mean (ng l−1) (SD) | Spiked post extraction mean (ng l−1) (SD) | Mean tofacitinib recovery (%) |
|---|---|---|---|
| 0.1 | 0.12 (0.02) | 0.11 (0.01) | 108.55 |
| 0.25 | 0.28 (0.01) | 0.25 (0.01) | 110.71 |
| 0.5 | 0.52 (0.04) | 0.49 (0.04) | 106.65 |
| 1 | 0.99 (0.08) | 0.89 (0.03) | 111.28 |
| 2.5 | 2.61 (0.13) | 2.41 (0.16) | 108.24 |
| 5 | 5.30 (0.22) | 4.82 (0.06) | 109.88 |
| 10 | 10.7 (0.75) | 9.91 (0.34) | 104.66 |
| 50 | 55.21 (3.83) | 50.20 (0.82) | 109.98 |
| 100 | 108.26 (4.57) | 100.39 (0.47) | 107.84 |
