Fig. 1.

Characterization of new patient-derived PA in vitro models DKFZ-BT308, DKFZ-BT314, and DKFZ-BT317. (A) t-SNE analysis of DNA methylation profiles of pediatric brain tumors (selected tumor types from mnp V12.3 reference set; www.molecularneuropathology.org), midline PA (PA_MID) and posterior fossa PA (PA_PF) enlarged on the right. Dashed circles: PA cell lines from p7 and p15 (passage 7 and 15), closed circles: corresponding primary samples. (B) MPAS signature ssGSEA scores. normal: normal cerebellum; PA: primary PA. ***Tukey multiple comparisons of means adjusted P-value: 2.7 × 10⁻⁶. Expression data: ps_mkheidel_mkdkfz209_u133p2. (C) Cell counts of PA cell lines in proliferation or OIS. DOX: doxycycline; DKFZ-BT66: historical data. (D) Western blot of SV40 TAg and p21^Cip1. OIS: 5 days of doxycycline (DOX) withdrawal. (E) ssGSEA z-scores of senescence gene sets. N = 3 independent expression samples per condition. DOX: doxycycline.