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. 2022 Oct 10;25(4):635–647. doi: 10.1093/neuonc/noac231

Figure 2.

Figure 2.

HOXA3 promotes GBM cell proliferation, tumor growth, and aerobic glycolysis. (A) Immunoblotting of HOXA3 in the indicated cells transduced with shHOXA3 or shRNA scramble. (B) Growth assay of the indicated GBM cells with HOXA3 knockdown or control. (C) Representative immunofluorescence images and quantifications of BrdU staining in GBM cells with HOXA3 knockdown or control. (D) Immunoblotting of cyclin E and CDK2 in the indicated GBM cells (up). Cell cycle analyses in GBM cells transduced with shHOXA3 or shRNA scramble (below). (E) Orthotopic tumor growth abilities of LN229 cells expressing Ctrl or shHOXA3 and survival rates of mice (n = 5). (F) Glycolytic rate of the indicated GBM cells transduced with shCtrl, shHOXA3, or shHOXA3/HOXA3. ECAR extracellular acidification rate. (G) Cellular oxygen consumption rate of the indicated GBM cells transduced with shCtrl, shHOXA3, or shHOXA3/HOXA3. All data were shown as the mean ± SD (n = 3–5), *P <.05, **P < .01.