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. 2022 Oct 10;25(4):635–647. doi: 10.1093/neuonc/noac231

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Figure 5. — HOXA3 directly interacts with KDM6A. (A) proximity ligation assay (PLA) assay of HOXA3-KDM6A expression in GBM cells using anti-HOXA3 and anti-KDM6A antibodies. (B) Immunoblot analysis of Co-IP of HOXA3 and KDM6A in extracts from LN229 and GBM01 cells. (C) Immunoblot analysis of Flag and HA in Flag-immunoprecipitated HOXA3 fragments from 293T cells expressing HOXA3-Flag and KDM6A-HA. (D) GST-pull-down analysis of the interaction between HOXA3 and KDM6A. (E) Immunoblot analysis of Co-IP of the interaction between different domains of HOXA3 and KDM6A. (F) Immunoblot analysis of Co-IP of the interaction between HOXA3 and different domains of KDM6A. (G) Molecular docking analysis of DUF domain of HOXA3 and TPR domain of KDM6A.