Figure 2.

Zinc deficiency was associated with biological processes and the KEGG pathway for hypoxia. Microarray analysis was performed to determine the zinc deficiency-induced differentially expressed genes in EMosis cells. (A-C) The top 10 enriched cellular components (CC), biological processes (BP), and KEGG pathways in ascending order of -log 10 (FDR p-value). The enriched CC contained nucleoplasm, nuclear parts, and nuclear lumen. The enriched BPs included responses to hypoxia and decreased oxygen levels. The enriched KEGG pathways included the HIF-1 signaling pathway. The gene expression of SRA1 and ARID1A was validated by RT-PCR (D, E). The EMosis cells treated with 5 μM TPEN for 4 h demonstrated significantly increased SRA1 mRNA expression and decreased ARID1A mRNA expression compared to the cells without TPEN (all P<0.05). KEGG: Kyoto Encyclopedia of Genes and Genomes; EMosis cells: endometrial epithelial cells; FDR: false discovery rate; HIF-1: hypoxia-inducible factor-1; SRA1: steroid receptor RNA activator 1; ARID1A: AT-rich interaction domain 1A; RT-PCR: real-time polymerase chain reaction.