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. 2023 Mar 15;13(3):976–991.

Figure 3.

Figure 3

CZT induces Par-4 secretion in normal/immortalized cells. A. CZT induces Par-4 secretion in normal/immortalized cells (MEFs, HEL, BEAS-2B) and mice but not in lung cancer cells. Cells were treated with vehicle (V) or CZT (at indicated nM concentrations) for 24 h. C57BL/6 mice were treated with V or CZT for 5 days. Aliquots of the conditioned medium (CM) from the cells or mouse plasma samples were then subjected to western blot analysis for Par-4. The corresponding gels were stained with Coomassie blue, and the intensity of Par-4 bands were normalized relative to albumin. Fold change in Par-4 in response to CZT relative to vehicle is shown. B. CZT induces Par-4 secretion in normal/immortalized cells, but several other drugs do not. HEL cells were treated with vehicle or the indicated drugs (at 500 nM) for 24 h. Aliquots of the CM from the cells were then subjected to western blot analysis for Par-4. The corresponding gels were stained with Coomassie blue, and the intensity of Par-4 bands were normalized relative to albumin. Fold change in Par-4 in response to each drug relative to vehicle is shown.