Fig. 6.

Sequence analysis and differential protein expression analysis of novel proteins. A shows the Venn diagram of the distribution of novel proteins with homology to proteins from the five diatoms (Thalassiosira oceanica, Phaeodactylum tricornutum, Fistulifera solaris, Pseudo-nitzschia multistriata and Fragilariopsis cylindrus). B and C show the sequence alignments for the domain of novel protein NG457 (B), NG36 (C) and the result of the phylogenetic analysis of homologous amino acid sequences from other diatom species. Numbers no the branches indicate posterior probability values and the scale bares indicate the number of expected changes per site along the branches. Accession numbers from NCBI for the analyzed amino acid sequences are also indicated. D shows the heatmap of novel gene expressions under different nutrient limitations