Table 1.

Recovery rate of FACS-iChip

Number	Media type	Culture time	Wells with visible colonies	Culture recovery rate (%)		Colony count	Culture retrieval rate (%)
1	Solid medium (LB)	30 days	257		66.93	139	54.09
2	Solid medium (LB)	30 days	242		63.02	123	50.83
3	Liquid medium (LB)	50 days	114		29.69	16	14.04
4	Liquid medium (LB)	50 days	152		39.58	57	37.50
5	Liquid medium (LB)	50 days	133		34.64	60	45.11
6	Solid medium (LB)	50 days	84		21.88	31	36.90
7	Solid medium (LB)	50 days	95		24.74	31	32.63
8	Solid medium (LB)	50 days	74		19.27	26	35.13

Culture recovery rate means the ratio of number of visible colony wells to total wells. Culture retrieval rate means the ratio of number of wells that can be subcultured to number of visible colony wells. The LB medium was used in both preliminary culture and subculture. And the cloudy liquid was regarded as visible colonies in liquid medium