Amphileptus paracarchesii sp. nov. from life (A–H, J, L–M) and after protargol impregnation (I, K, N). A, B Right side view, arrows point to the groove, arrowhead shows the curved anterior body end. C Nuclear apparatus, arrowheads denote the four macronuclear nodules. D, G, J Shape variants, arrowheads mark the macronuclear nodules, arrow denotes the posterior groove. E Detail showing the apical group of extrusomes (arrow). F Detail showing the lateral groove situated in the posterior body region (arrow). H Contractile vacuoles (arrowheads). I Detail of the oral apparatus, showing a single perioral kinety right and left of the oral slit. K, N Detail of the anterior body portion, showing the ciliary pattern of the right and left sides of the holotype specimen. L A contracted individual, arrow shows the curved anterior body end. M Cytoplasmic extrusomes (arrowheads). Abbreviations: PK1, perioral kinety 1. PK2, perioral kinety 2. Scale bars = 100 μm