Figure 6. Prdm6 depletion inhibits a ductus-selective gene program.

(A) Principal component analysis of RNA-Seq data from ascending aorta and DA samples isolated from Wnt1^Cre2 Prdm6^fl/fl and littermate control mice at E18.5. (B) Two-dimensional hierarchical clustering of the top 1,000 genes differentially expressed in ascending aorta and DA samples from control and Prdm6-deficient mice. Potentially relevant Prdm6-dependent genes are listed on the right, and full gene lists for each cluster are available in Supplemental Table 2. (C) Volcano plot demonstrating differential gene expression between control and Prdm6-deficient DA samples. Genes colored in red are considered as significantly different (Padj < 0.01, logFC > 0.5, or logFC < –0.5). (D) Gene ontology analysis of genes significantly up- or downregulated by Prdm6 depletion in DA samples. logFC, log fold-change.