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. Author manuscript; available in PMC: 2023 Apr 6.
Published in final edited form as: Nat Aging. 2022 May 16;2(6):484–493. doi: 10.1038/s43587-022-00220-0

Table 1 |.

growth factors used for AFe differentiation.

Growth factor Manufacturer, catalog no. Days 0–1 Days 1–3 Days 3–4 Days 4–6
Wnt3a R&D Systems, 5036-GMP 50 ng ml−1 - - -
Activin A R&D Systems, 338-AC 100 ng ml−1 100 ng ml−1 - -
Dorsomorphin Torcis, 3093 - - 1μM -
SB431542 Torcis, 1614 - - 5μM 5μM
IWP2 Torcis, 3533 - - - 1μM