Fig. 2.

Transient expression of the chimeric fusion genes of HPV16 L1 protein in N. benthamiana. The indicated constructs were transiently expressed in N. benthamiana by Agrobacterium-mediated infiltration. Total soluble protein extracts were prepared from leaf tissues harvested at 4 dpi and separated by 10% SDS-PAGE. The gel was stained with Coomassie brilliant blue (CBB). Alternatively, the proteins on the gel were transferred onto a PVDF membrane for western blot analysis using anti-rabbit HPV16 L1 antibodies. M molecular weight standard; the arrow indicates chimeric fusion protein and cleaved L1