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. 2023 Apr 6:1–24. Online ahead of print. doi: 10.1007/s12026-023-09374-4

Table 2.

Predicted CTL epitopes from A. baumannii LPS assembly protein (LptD and LptE) to design multi-epitope vaccine construct with their corresponding MHC class I alleles and their immunogenic properties

Uniprot_ID CTL Epitope Alleles Position Score Antigencity Score Immunogenicity Conservancy Toxicity Hydrophobicity Hydropathicity Hydrophilicity Charge Mol wt.
V5VH20 TDDLETQLK HLA-A*01:01 50 2.907 1.1947 0.02584 100% Non-toxin −0.35 −1.3 0.87 −2 1062.27
GLVGCGFHL HLA-A*02:01 19 0.514 1.3983 0.1446 100% Non-toxin 0.26 1.41 −1.01 0.5 902.22
GTNPTATPL HLA-A*03:01 29 7.505 0.8191 0.08765 100% Non-toxin −0.04 −0.4 −0.37 0 871.08
LRLTVTFQI HLA-A*24:02 98 3.932 1.6349 0.14332 100% Non-toxin 0.01 1.08 −0.78 1 1090.47
SAGLVGCGF HLA-A*26:01 17 7.792 0.9454 0.04624 100% Non-toxin 0.24 1.46 −0.78 0 810.06
VLLRLTVTF HLA-B*08:01 96 1.293 0.6513 0.13174 100% Non-toxin 0.13 1.86 −0.97 1 1061.47
B0VMX7 QSDEIDYNL HLA-A*01:01 168 0.662 1.4015 0.25153 100% Non-toxin −0.26 −1.26 0.42 −3 1096.24
KSAPEAQLR HLA-A*03:01 68 1.182 1.3792 0.03163 100% Non-toxin −0.39 −1.16 0.74 1 999.25
VLAVPYFNF HLA-A*24:02 258 0.376 2.0269 0.13498 100% Non-toxin 0.27 1.47 −1.38 0 1069.39
YDATITPRY HLA-A*26:01 303 0.676 1.4273 0.23878 100% Non-toxin −0.21 −0.81 −0.19 0 1099.32
LPQFLLNYV HLA-B*07:02 437 1.514 1.1172 0.05134 100% Non-toxin 0.15 0.94 −1.26 0 1106.47
MLQGEFRYL HLA-B*08:01 318 1.555 0.4306 0.24572 100% Non-toxin −0.11 −0.1 −0.39 0 1156.5
GRTVRADKV HLA-B*27:05 135 3.095 1.4877 0.03409 100% Non-toxin −0.45 −0.81 0.9 2 1001.27
DKSAPEAQL HLA-B*39:01 67 0.367 1.1392 0.03446 100% Non-toxin −0.27 −1.04 0.74 −1 958.15
VKDIPVLAV HLA-B*40:01 253 3.103 1.0029 0.18268 100% Non-toxin 0.14 1.52 −0.29 0 953.32
LSVPVYLNL HLA-B*58:01 291 0.371 1.2858 0.02582 100% Non-toxin 0.19 1.4 −1.13 0 1017.37
QTLTPRAFY HLA-B*15:01 550 1.025 0.8494 0.1748 100% Non-toxin −0.16 −0.43 −0.52 1 1096.37