. 2023 Apr 6:1–24. Online ahead of print. doi: 10.1007/s12026-023-09374-4

Table 3.

Predicted HTL epitopes from A. baumannii LPS assembly protein (LptD and LptE) to design multi-epitope vaccine construct with their corresponding MHC class II alleles and their immunogenic properties

Uniprot_ID	MHC II epitope	Alleles	Pos	IC50 value	Percentile_Rank	Antigencity score	Conservancy	Toxicity	Hydrophobicity	Hydropathicity	Hydrophilicity	Charge	Mol wt.
V5VH20	LRLTVTFQIEDRQGN	HLA-DPA101:03, HLA-DPB102:01, HLA-DPB101:01, HLA-DRB101:01, HLA-DRB109:01,HLA-DRB302:02, HLA-DRB113:02, HLA-DRB111:01, HLA-DRB104:01, HLA-DRB112:01, HLA-DPA103:01, HLA-DPB104:02, HLA-DRB104:05, HLA-DRB115:01, HLA-DQA101:01, HLA-DQB105:01, HLA-DRB108:02, HLA-DPA102:01, HLA-DPB114:01, HLA-DPB104:01, HLA-DQA105:01, HLA-DQB103:01, HLA-DQA104:01, HLA-DQB104:02, HLA-DPA102:01, HLA-DPA102:01, HLA-DPB105:01, HLA-DPA101:03, HLA-DQA105:01, HLA-DQB102:01, HLA-DQA103:01, HLA-DQB103:02, HLA-DQA101:02, HLA-DQB106:02, HLA-DRB301:01, HLA-DRB501:01, HLA-DRB107:01, HLA-DRB401:01, HLA-DRB1*03:01	98–112	73	1.9	1.5395	100%	Non-toxin	−0.28	−0.61	0.16	0	1790.24
B0VMX7	VVVPQFTLDTGLNFE	HLA-DRB115:01, HLA-DRB301:01, HLA-DPA103:01, HLA-DPB104:02, HLA-DPA101:03, HLA-DPB102:01,HLA-DRB101:01, HLA-DRB109:01,HLA-DRB302:02, HLA-DRB113:02, HLA-DRB111:01, HLA-DRB104:01, HLA-DRB112:01,HLA-DRB104:05, HLA-DQA101:01, HLA-DQB105:01, HLA-DRB108:02, HLA-DPA102:01, HLA-DPB114:01, HLA-DPA101:03, HLA-DPB104:01, HLA-DQA105:01, HLA-DQB103:01, HLA-DQA104:01, HLA-DQB104:02, HLA-DPA102:01, HLA-DPB101:01, HLA-DPA102:01, HLA-DPB105:01,HLA-DQA105:01, HLA-DQB102:01, HLA-DQA103:01, HLA-DQB103:02, HLA-DQA101:02, HLA-DQB106:02, HLA-DRB501:01, HLA-DRB107:01, HLA-DRB401:01, HLA-DRB1*03:01	529–543	50	0.28	1.2627	100%	Non toxin	0.06	0.56	−0.5	−2	1679.13

Uniprot_ID

MHC II epitope

Alleles

Pos

IC50 value

Percentile_Rank

Antigencity score

Conservancy

Toxicity

Hydrophobicity

Hydropathicity

Hydrophilicity

Charge

Mol wt.

V5VH20

LRLTVTFQIEDRQGN

HLA-DPA1*01:03, HLA-DPB1*02:01, HLA-DPB1*01:01, HLA-DRB1*01:01, HLA-DRB1*09:01,HLA-DRB3*02:02, HLA-DRB1*13:02, HLA-DRB1*11:01, HLA-DRB1*04:01, HLA-DRB1*12:01, HLA-DPA1*03:01, HLA-DPB1*04:02, HLA-DRB1*04:05, HLA-DRB1*15:01, HLA-DQA1*01:01, HLA-DQB1*05:01, HLA-DRB1*08:02, HLA-DPA1*02:01, HLA-DPB1*14:01, HLA-DPB1*04:01, HLA-DQA1*05:01, HLA-DQB1*03:01, HLA-DQA1*04:01, HLA-DQB1*04:02, HLA-DPA1*02:01, HLA-DPA1*02:01, HLA-DPB1*05:01, HLA-DPA1*01:03, HLA-DQA1*05:01, HLA-DQB1*02:01, HLA-DQA1*03:01, HLA-DQB1*03:02, HLA-DQA1*01:02, HLA-DQB1*06:02, HLA-DRB3*01:01, HLA-DRB5*01:01, HLA-DRB1*07:01, HLA-DRB4*01:01, HLA-DRB1*03:01

98–112

1.9

1.5395

100%

Non-toxin

−0.28

−0.61

0.16

1790.24

B0VMX7

VVVPQFTLDTGLNFE

HLA-DRB1*15:01, HLA-DRB3*01:01, HLA-DPA1*03:01, HLA-DPB1*04:02, HLA-DPA1*01:03, HLA-DPB1*02:01,HLA-DRB1*01:01, HLA-DRB1*09:01,HLA-DRB3*02:02, HLA-DRB1*13:02, HLA-DRB1*11:01, HLA-DRB1*04:01, HLA-DRB1*12:01,HLA-DRB1*04:05, HLA-DQA1*01:01, HLA-DQB1*05:01, HLA-DRB1*08:02, HLA-DPA1*02:01, HLA-DPB1*14:01, HLA-DPA1*01:03, HLA-DPB1*04:01, HLA-DQA1*05:01, HLA-DQB1*03:01, HLA-DQA1*04:01, HLA-DQB1*04:02, HLA-DPA1*02:01, HLA-DPB1*01:01, HLA-DPA1*02:01, HLA-DPB1*05:01,HLA-DQA1*05:01, HLA-DQB1*02:01, HLA-DQA1*03:01, HLA-DQB1*03:02, HLA-DQA1*01:02, HLA-DQB1*06:02,

HLA-DRB5*01:01, HLA-DRB1*07:01, HLA-DRB4*01:01, HLA-DRB1*03:01

529–543

0.28

1.2627

100%

Non toxin

0.06

0.56

−0.5

−2

1679.13