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Comparison of FLAM‐Seq, PAIso‐Seq, and SM‐PAT protocols for mRNA poly(A) tail sequencing with the PacBio platform. Violet hatched lines indicate the G/I tag sequence. Red hatched lines indicate the 3′ adapter sequence. Orange hatched lines indicate the 5′ adapter sequence. The red line indicates the oligo(dT) sequence. dU nucleotides are marked in black.
