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Schematic view of the Ac‐IPG approach. (A) Functional design of the Ac‐IPG‐PNP tag (13C isotope locations of the triplex Ac‐IPG‐PNP tag are marked with asterisk); (B) triplex isobaric labeling steps; (C) LC‐MS for a mixture of triplex labeled samples. LC‐MS, liquid chromatography mass spectrometry [Color figure can be viewed at wileyonlinelibrary.com]
