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. 2023 Apr 6;18(4):e0283894. doi: 10.1371/journal.pone.0283894

Fig 5. Immunohistochemical analysis of RNF43 antibodies.

Fig 5

The indicated cell lines were formalin-fixed and embedded in paraffin. HCT116 cells transiently overexpressing RNF43 were also included. All antibodies give the same non-specific staining pattern as observed in the IF-experiments, showing that they all strongly recognize a non-specific protein not being RNF43. The 8D6 and HPA008079 antibodies can detect overexpressed RNF43, indicated by arrows, while this is not the case for ab84125 and ab217787. Scale bar, 50um.