Table 3.
Performance metrics of the N200 VOC assay for each of the probes in singleplex, duplex or triplex.
| Assay | Slope | y-intercept | Efficiency | R2 | |
|---|---|---|---|---|---|
| Universal | Singleplex | −3.347 | 40.236 | 98.9 | 0.990 |
| Duplex (with R203M) | −3.366 | 39.50 | 98.2 | 0.991 | |
| Duplex (with R203K + G204R) | −3.299 | 38.793 | 101.0 | 0.990 | |
| Duplex (with S202S) | −3.311 | 40.207 | 100.5 | 0.990 | |
| Duplex (with T205I) | −3.461 | 39.773 | 94.5 | 0.990 | |
| Triplex (with R203M, R203K + G204R) | −3.443 | 39.661 | 95.2 | 0.995 | |
| R203K + G204R (Omicron/Alpha) |
Singleplex | −3.429 | 38.834 | 95.7 | 0.998 |
| Duplex (with Universal) | −3.334 | 39.585 | 99.5 | 0.991 | |
| Triplex (with Universal, R20K + G204R) | −3.464 | 39.902 | 94.4 | 0.995 | |
| T205I (Beta) |
Singleplex | −3.396 | 39.951 | 97.0 | 0.997 |
| Duplex (with Universal) | −3.533 | 39.952 | 91.9 | 0.994 | |
| S202S (Gamma) |
Singleplex | −3.249 | 40.363 | 103.1 | 0.998 |
| Duplex (with Universal) | −3.352 | 39.891 | 98.8 | 0.988 | |
| R203M (Delta) |
Singleplex | −3.541 | 40.365 | 91.6 | 0.998 |
| Duplex (with Universal) | −3.387 | 39.513 | 97.4 | 0.990 | |
| Triplex (with Universal, R203M) | −3.442 | 39.124 | 95.2 | 0.995 | |