Table 5.
Gene copies/μL of RNA (mean ± SD; n = 3) detected by the Universal, R203M, or R203K + G204R probes in the N200 assay (triplexed) when tested against TWIST controls for variants of SARS-CoV-2 using RT-dPCR.
| Dilution from stock (~1 ∗ 10^4 copies/μL) | Universal (all) | R203K + G204R (Omicron/Alpha) |
R203M (Delta) |
|
|---|---|---|---|---|
| Twist 23 (Delta) | 5× | 2769 ± 173 | 0 ± 0 | 2767 ± 173 |
| 500× | 25 ± 2 | 0 ± 0 | 25 ± 2 | |
| 5000× | 3 ± 1 | 0 ± 0 | 3 ± 1 | |
| Twist 48 (Omicron) | 5× | 824 ± 12 | 820 ± 22 | 2 ± 2 |
| 50× | 78 ± 3 | 77 ± 3 | 0 ± 0 | |
| 500× | 5 ± 0 | 5 ± 0 | 0 ± 0 |