REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-human IgG1	SouthernBiotech	Cat# 9052-09, RRID:AB_2796621
anti-human IgG3	SouthernBiotech	Cat# 9210-09, RRID:AB_2796701
anti-human IgM	SouthernBiotech	Cat# 9020-09, RRID:AB_2796577
anti-human IgA1	SouthernBiotech	Cat# 9130-09, RRID:AB_2796656
anti-human CD66b	BioLegend	Cat# 305112, RRID:AB_2563294
anti-guinea pig C3	MP Biomedicals	Cat# 0855385, RRID:AB_2334913
anti-human CD107a	BioLegend	Cat# 328634, RRID:AB_2563851
anti-human CD56	BD Biosciences	Cat# 335791, RRID:AB_399970
anti-human CD3	BioLegend	Cat# 300426, RRID:AB_830755
anti-human MIP-1β	BD Biosciences	Cat# 562900, RRID:AB_2737877
anti-human IFNg	BD Biosciences	Cat# 562900, RRID:AB_2737877
anti-human CD32 (FcγRIIA)	Bio X Cell	Cat# BE0224, RRID:AB_2687707
anti-human CD16 (FcγRIII)	Bio-Rad	Cat# MCA1193GA, RRID:AB_324304
Chemicals, peptides, and recombinant proteins
SARS-CoV-2 Spike S1+S2 (D614G) trimer Protein (ECD, His tag)	SinoBiological	Cat: 40589-V08H8, YP_009724390.1
SARS-CoV-2 B.1.1.7 Spike S1+S2 trimer Protein (ECD, His tag)	SinoBiological	Cat: 40589-V08H12, YP_009724390.1
SARS-CoV-2 B.1.351 Spike S1+S2 trimer Protein (ECD, His tag)	SinoBiological	Cat: 40589-V08H13, YP_009724390.1
SARS-CoV-2 B.1.617.2 Spike S1+S2 trimer Protein (ECD, His tag)	SinoBiological	Cat: 40589-V08H10, YP_009724390.1
Human Fc receptors	Produced at the Duke Human Vaccine Institute	NA
FIX&Perm Cell Permeabilization Kit	Thermo Fisher Scientific	Cat: GAS004
EDC	Thermo Fisher Scientific	Cat: A35391
Sulfo-NHS	Thermo Fisher Scientific	Cat: A39269
EZ-link™ Sulfo-NHS-LC-LC-Biotin	Thermo Fisher Scientific	Cat: A35358
FluoSphere™ NeutrAvidin™ conjugated beads	Thermo Fisher Scientific	Cat: F8776 and F8775
Lyophilized Guinea Pig complement	Cedarlane	Cat: CL4051
Gelatin veronal buffer	Sigma-Aldrich	Cat: G6514
GolgiStop	BD Biosciences	Cat: E1483
4% Paraformaldehyde	SantaCruz Biotechnology	Cat: Sc-281692
Ammonium-Chloride-Potassium (ACK) buffer	VWR	Cat: 10128-802
Critical commercial assays
RosetteSep Human NK Cell Enrichment Cocktail	Stem Cell Technologies	Cat: 15065
Experimental models: Cell lines
THP-1 cells	ATCC	CAT#: TIB-202 RRID: CVCL_0006
Expi293F cells	Thermo Fisher Scientific	Cat: A14527, RRID:CVCL_D615
Recombinant DNA
Mammalian cell expression vector pCMV-IRES-puro	Codex BioSolutions, Inc
Software and algorithms
Intellicyt ForeCyt Software	Sartorious	https://intellicyt.com/products/software/
R programming language	https://www.r-project.org/	Version 4.0.1
R Studio	https://www.rstudio.com/	Version 1.3.1093
Prism 9	Graph Pad	Version 9.3.1