Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Apr 6;11(7):e15652. doi: 10.14814/phy2.15652

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Effects of probenecid on sodium and fluid transport in mpkCCD_cl4 cells. (a) Accumulation of ATP in the apical chamber after 1‐h mechanical stimulation with rocking and treatment with vehicle and various concentrations of probenecid. (b) Dose‐dependent effect of probenecid on ENaC‐mediated sodium reabsorption. Amiloride‐sensitive current was measured with an epithelial Volt/Ohm meter after 8‐h‐long treatment with probenecid. (c) Effect of apical application of 100 μM ATP on ENaC‐depended I_sc without probenecid and in the presence of the drug. (d) mpkCCD_cl4 cells grown in 3D culture in the presence of forskolin form cysts. (e) Median cyst size in the control‐ and probenecid‐treated wells on summary graph. (f) Cyst growth in Matrigel in presence of 10 μM amiloride. Each dot represents one cyst size, grouped by wells. Columns are mean ± SEM of the three medians in the wells treated with vehicle, probenecid, or amiloride. *p < 0.05 per unpaired t‐test.