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. 2023 Mar 2;42(15):1224–1232. doi: 10.1038/s41388-023-02638-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Rare codons are equally enriched in NRAS and KRAS in humans. B Schematic for absolute quantitation of Ras protein abundance. Coomassie blue staining of 200 ng of isotope labelled “heavy” Ras protein standards indicates high purity suitable for precise quantitation and ratiometric comparison with endogenous Ras proteins.