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. 2023 Feb 23;37(4):820–834. doi: 10.1038/s41375-023-01854-8

Fig. 3. siRNA-LNP provide stringent gene knockdown in cell lines and AML blast.

Fig. 3

a–f Kasumi-1 cells were treated with 2 µg/ml siRNA-LNPs for 24 h then washed thrice in PBS. The knockdown of RUNX1/ETO relative to GAPDH on day 3 at the transcript level (a) (n = 5) and in western blotting (b). c Proliferation curve of Kasumi-1 cells following siRNA-LNPs treatment (n = 3). d Cell cycle profile of Kasumi-1 cells on day 6. e Quantification of senescent cells on day 6 by beta galactosidase staining (n = 3). f Colony formation units of Kasumi-1 cells in first and second platings (n = 3). g Schematic illustration of AML blast co-culture on MSCs feeders and treatment with siRNA-LNPs. h RUNX1/ETO expression relative to GAPDH in different t(8;21) AML blasts following siRNA-LNPs treatment. i Western blotting showing RUNX1/ETO, RUNX1, CCND2 and GAPDH in two AML samples after siRNA-LNPs treatment. Significance was tested by unpaired Student’s t tests (a, c, e, f).