KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-BCL2 | Proteintech | Cat#12789-1-AP; RRID:AB_2227948 |
| anti-LC3B | Novus Biologicals | Cat#NB100-2200; RRID:AB_10003146 |
| Contlol mouse IgG | Thermo Fisher Scientific | Cat#10400C; RRID:AB_2532980 |
| anti-ITGB1 (AIIB2 clone) | Thermo Fisher Scientific | Cat#605-930 |
| anti-fibronectin/FN1 | Thermo Fisher Scientific | Cat#PA5-29578; RRID:AB_2547054 |
| anti-p-BCL2 (Ser70) | Santa Cruz Biotechnology | Cat#sc-293128 |
| anti-BECN1 | Santa Cruz Biotechnology | Cat#sc-48341; RRID:AB_626745 |
| anti-integrin α5 (ITGA5) | Santa Cruz Biotechnology | Cat#sc-376199; RRID:AB_10987904 |
| anti-integrin β1 (ITGB1) | Santa Cruz Biotechnology | Cat#sc-374429; RRID:AB_11012020 |
| anti-p-FAK (Tyr397) | Santa Cruz Biotechnology | Cat#sc81493; RRID:AB_1125825 |
| anti-Sec6 | Santa Cruz Biotechnology | Cat#sc-374054; RRID:AB_10916711 |
| Horseradish peroxidase (HRP)-conjugated anti-β-actin | Santa Cruz Biotechnology | Cat#sc-47778 HRP; RRID:AB_2714189 |
| anti-α-tubulin | Santa Cruz Biotechnology | Cat#sc-53029; RRID:AB_793541 |
| anti-SQSTM1/p62 | Cell Signaling Technology | Cat#23214; RRID:AB_2798858 |
| anti-Akt (pan) | Cell Signaling Technology | Cat#4691; RRID:AB_915783 |
| anti-phospho-Akt (Ser473) | Cell Signaling Technology | Cat#4060; RRID:AB_2315049 |
| anti-IKKα | Cell Signaling Technology | Cat#11930; RRID:AB_2687618 |
| anti-IKKβ | Cell Signaling Technology | Cat#8943; RRID:AB_11024092 |
| anti-phospho-IKKα/β (Ser176/180) | Cell Signaling Technology | Cat#2697; RRID:AB_2079382 |
| anti-JNK | Cell Signaling Technology | Cat#9252; RRID:AB_2250373 |
| anti-phospho-JNK (Thr183/185) | Cell Signaling Technology | Cat#4668; RRID:AB_823588 |
| anti-JNK1 | Cell Signaling Technology | Cat#3708; RRID:AB_1904132 |
| anti-JNK2 | Cell Signaling Technology | Cat#9258; RRID:AB_2141027 |
| anti-FAK | Cell Signaling Technology | Cat#3285; RRID:AB_2269034 |
| anti-phospho-Src (Tyr416) | Cell Signaling Technology | Cat#6943; RRID:AB_10013641 |
| anti-Src | Cell Signaling Technology | Cat#2123; RRID:AB_2106047 |
| anti-TBC1D1 | Cell Signaling Technology | Cat#4629; RRID:AB_1904162 |
| anti-AS160 | Cell Signaling Technology | Cat#2670; RRID:AB_2199375 |
| anti-ATG7 | Cell Signaling Technology | Cat#8558; RRID:AB_10831194 |
| anti-AMPK | Cell Signaling Technology | Cat#2532; RRID:AB_330331 |
| anti-phospho-AMPK (Thr172) | Cell Signaling Technology | Cat#2535; RRID:AB_331250 |
| HRP-conjugated anti-mouse IgG light-chain specific | Cell Signaling Technology | Cat#91196 |
| HRP-conjugated anti-rabbit IgG conformation specific | Cell Signaling Technology | Cat#5127; RRID:AB_10892860 |
| HRP-conjugated anti-rat IgG | Cell Signaling Technology | Cat#7077; RRID:AB_10694715 |
| HRP-conjugated anti-rabbit IgG | Jackson ImmunoResearch | Cat#111-035-033; RRID:AB_2313567 |
| HRP-conjugated anti-mouse IgG | Jackson ImmunoResearch | Cat#115-035-003; RRID:AB_10015289 |
| Bacterial and Virus Strains | ||
| Adeno-associated virus (AAV): AAV2/9-shFn1 | This paper | N/A |
| AAV: AAV2/8-shItga5 | This paper | N/A |
| AAV: AAV2/8-shAtg7 | This paper | N/A |
| AAV: AAV2/8-TBG-PI-Null-bGH | Gift of James M. Wilson lab | Addgene viral prep Cat#105536-AAV8; RRID:Addgene_105536 |
| Lentivirus: mCherry-EGFP-LC3 | This paper | N/A |
| Lentivirus: shRNA (pLKO.1) | This paper | N/A |
| Lentivirus: shRNA (tet-pLKO) | This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Insulin | Millipore Sigma | Cat#I0516-5ML; CAS: 11070-73-8 |
| Heparinized microvette | Braintree Scientific | Cat#MV-CB300 16443 |
| anti-BCL2 antibody-conjugated agarose beads | Santa Cruz Biotechnology | Cat#sc-7382 AC |
| Protein A/G PlusAgarose | Santa Cruz Biotechnology | Cat#sc-2003 |
| Lipofectamine 3000 | Thermo Fisher Scientific | Cat#L3000150 |
| Polybrene | Millipore Sigma | Cat#TR-1003-G |
| Fibronectin (FN1) | Millipore Sigma | Cat#F2006; EC: 289-149-2 |
| Bovine Serum Albumin (BSA) solution | Millipore Sigma | Cat#A1595; CAS: 9048-46-8 |
| AICAR | Cayman Chemical | Cat#10010241; CAS:2627-69-2 |
| Chloroquine (phosphate) | Cayman Chemical | Cat#14194; CAS:50-63-5 |
| Halt Protease Inhibitor Cocktail | Thermo Fisher Scientific | Cat#78438 |
| ProLong Diamond Antifade Mountant | Thermo Fisher Scientific | Cat#P36961 |
| Critical Commercial Assays | ||
| Proteome Purify 2 Mouse Serum Protein | R&D systems | Cat#MIDR002-020 |
| Mouse Fibronectin ELISA Kit | Abcam | Cat#ab210967 |
| High-Capacity cDNA Reverse Transcription Kit | Thermo Fisher Scientific | Cat#4368814 |
| PowerUP SYBR Green Master Mix | Thermo Fisher Scientific | Cat#A25742 |
| BCA protein assay kit | Thermo Fisher Scientific | Cat#23225 |
| Experimental Models: Cell Lines | ||
| Mouse: C2C12 cells | ATCC | Cat#CRL-1772 |
| Human: HeLa cells | ATCC | Cat#CCL-2 |
| Human: 293T cells | ATCC | Cat#CRL-3216 |
| Human: Huh7 cells | JCRB cell bank | Cat#JCRB0403 |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6J mice | The Jackson Laboratory | Cat#000664; RRID:IMSR_JAX:000664 |
| Mouse: GFP-LC3 mice: Tg(CAG-EGFP/Map1lc3b)53Nmz | RIKEN BioResource Research Center | Cat#RBRC00806 |
| Oligonucleotides | ||
| Target sequences for shRNA, see Table S1 | This paper | N/A |
| Primers for qPCR, see Table S2 | This paper | N/A |
| Recombinant DNA | ||
| pCDH-CMV-MCS-EF1-Puro | System Biosciences | Cat#CD510B-1 |
| pCDH-mCherry-EGFP-LC3 | This paper | N/A |
| pLKO.1-TRC cloning vector | Gift of David Root lab58 | Addgene plasmid Cat# 10878; RRID:Addgene_10878 |
| Tet-pLKO-puro | Gift of Dmitri Wiederschain lab59 | Addgene plasmid # 21915; RRID:Addgene_21915 |
| pLKO.1 scramble shRNA | Gift of David Sabatini lab60 | Addgene plasmid # 1864; RRID:Addgene_1864 |
| tet-pLKO-puro-Scrambled | Gift of Charles Rudin lab61 | Addgene plasmid # 47541; RRID:Addgene_47541 |
| pCMV-VSV-G | Gift of Bob Weinberg lab62 | Addgene plasmid # 8454; RRID:Addgene_8454 |
| psPAX2 | Gift of Didier Trono lab | Addgene plasmid # 12260; RRID:Addgene_12260 |
| pAAV-MHCK7-miR30-mCherry-shFn1 | This paper | N/A |
| pAAV-TBG-miR30-mCherry-shItga5 | This paper | N/A |
| pAAV-TBG-miR30-mCherry-shAtg7 | This paper | N/A |
| Software and Algorithms | ||
| ImageJ | National Institutes of Health (NIH) | https://imagej.nih.gov/ij/ |
| NIS-Elements | Nikon | https://www.microscope.healthcare.nikon.com/products/software/nis-elements |
| BellCurve for Excel | Social Survey Research Information Co. | https://bellcurve.jp/ex/ |
| BioRender | BioRender | https://biorender.com/ |
| Deposited Data | ||
| Source data for all figures, see Data S1 | This study | N/A |