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. Author manuscript; available in PMC: 2023 Apr 15.
Published in final edited form as: J Mol Biol. 2023 Feb 16;435(8):168010. doi: 10.1016/j.jmb.2023.168010

Figure 5.

Figure 5.

Assessing the level of activated SHP2 in cells using Mb11 and Mb13. (a) The amounts of SHP2 in HEK293 cells that were captured using Mb11 or Mb13 with and without EGF stimulation (5 min with 50 ng/ml). The captured SHP2 protein was detected using an anti-SHP2 antibody. Purified SHP2 was included as a control in lane C. (b) Quantification of captured SHP2 using Mb11 or MB13 as a capture reagent in the absence or presence of GAB2 pY peptide using flow cytometry. The raw data are provided in Supplementary Figure 5.