Table 3.

List of the most abundant aptamer sequences obtained after SELEX against Hsp70.

Apt ID	Sequence 5′→ 3′ with primer region underlined	NGS (%)
HSe-1[a, b]	GCAGCAGAGATAGACGCTATTTCTTA*GA*CCTTTCTA*A*TTTA*A*CTA*CTCA*A*TGGCA*TA*GCTGGCA*C	8.37
HSe-2[a, b]	GCAGCAGAGATAGACGCTATTCTCTA*GA*CCTTCTA*A*TTTA*CA*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C	4.57
HSe-3[a, b]	GCAGCAGAGATAGACGCTAA*GGGA*A*GTA*GGA*A*GA*A*GGGA*TGCCCTGCA*A*TGGCA*TA*GCTGGCA*C	1.58
HSe-4[a, b]	GCAGCAGAGATAGACGCTAGGA*TCTCA*TA*GTTCTTGTA*A*TGA*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C	1.47
HSe-5[a, b]	GCAGCAGAGATAGACGCTAA*TCGA*TGA*GA*CCTTCTA*A*TTA*A*CTA*TTCCA*A*TGGCA*TA*GCTGGCA*C	0.78
HSe-6[a, b]	GCAGCAGAGATAGACGCTATCTCTTA*GA*CCTTCTA*A*TTTA*A*CTA*CTCA*A*TGGCA*TA*GCTGGCA*C	0.74
HSe-7[a, b]	GCAGCAGAGATAGACGCTAGA*TCTCCA*GTCCTTCTA*A*TTA*A*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C	0.43
HSe-8[a, b]	GCAGCAGAGATAGACGCTAGTCCTCA*TA*GGCCTTCTA*A*TTA*A*CTA*TTCA*A*TGGCA*TA*GCTGGCA*C	0.21
HSe-9[a,b]	GCAGCAGAGATAGACGCTAA*GA*CCTCA*TA*GTCCTTCTA*A*TTA*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C	0.12
HSc-2[c]	GCAGCAGAGATAGACGCTATTCTCTA*GA*CCTTCTA*A*TTTA*CA*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C
HSc-9[c]	GCAGCAGAGATAGACGCTAA*GA*CCTCA*TA*GTCCTTCTA*A*TTA*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C
HSc-9.1[c]	A*GA*CCTCA*TA*GTCCTTCTA*A*TTA*TA*CTCCA*A*TGGCA*TA*GCTGGCA*C

^[a] Reverse complement sequence purchased 5′-biotinylated; ^[b] 5′-6-FAM-labelled reverse primer used for PEX production of dA^*-modified strand; ^[c] aptamers synthesised both with and without 5′-6-FAM label; HSe enzymatically synthesised; HSc chemically synthesised.