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. 2023 Apr 6;6:65. doi: 10.1038/s42004-023-00862-0

Table 6.

Binding affinities of aptamers and their scrambled alternatives with His-Hsp70.

Aptamers BLI [Kd] Plate assay [Kd]
HSc-2 66 ± 4 nM 245 ± 6 nM
HSNat-2 >5 µM >10 µM
HSc-9 36 ± 2 nM 29.1 ± 1.4 nM
HSNat-9 4.7 ± 0.4 µM >10 µM
HSc-9.1 29.1 ± 2 nM 14.8 ± 0.3 nM
Scr1 560 ± 15 nM 536 ± 35 nM
Scr2 620 ± 55 nM 687 ± 43 nM
Scr3 700 ± 37 nM 775 ± 21 nM