Fig. 5.

Effects of Tre–car on Nt and PARP. A substantial increase in Nt-positive staining was observed in spinal cord tissues collected from mice 24 h after SCI compared to sham mice (B, A). Treatment with Tre and Car reduced the positive staining of Nt (C, D). Tre–car treatment protects tissue after SCI (E). A substantial increase in PAR-positive staining was observed in spinal cord tissues of SCI mice compared to sham mice (H, G). Treatment with Tre and Car reduced the positive staining of PARP (I, J). Tre–car treatment protects tissue after SCI (K). Quantitative panels of Nt and PARP, respectively (F, L). 8-hydroxy-2-deoxyguanosine (8-OHdG) content in serum (M). ***p < 0.001 versus sham; ^#p < 0.05 versus SCI; ^##p < 0.01 versus SCI; ^###p < 0.001 versus SCI; ND not detectable. (samples n = 10, three individual experiments). Data are expressed as SD