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3D reconstruction of a simulated biological vesicle. (a–c) Three 10-voxel-thick central slices of the vesicle model in the XY, XZ and YZ planes, respectively. The corresponding three reconstructed slices with FBP (d–f), SIRT (g–i), GENFIRE (j–l), and RESIRE (m–o), where the missing wedge direction is along the z-axis. (p) The FSC between the model and the reconstructions showing that RESIRE produces a better reconstruction than FBP, SIRT, and GENFIRE at all spatial frequencies.
