FIGURE 2.

Statistical investigation of different body parameters to characterize obesity. (A) Body weight (g), (B) visceral, and (C) subcutaneous fat weight (g) of female (red) and male (blue) mice at age of 8, 24, and 40 weeks were analyzed at the end of the experiment. The group size was as follows: n(wt) = 45 (A), 41 (B), 33 (C), n(ob/ob) = 42 (A,C), 45 (B). Significances of differences between the groups were tested by three-way ANOVA. The analysis revealed a genotype and age, but no sex effect for all three examined parameters. Statistical significance was set at ****p < 0.0001 for genotype effect and ^##p < 0.01 or ^####p < 0.0001 for age effect. ob/ob, leptin deficient mice; wt, wild type; w, weeks (these abbreviations will be consistently used in all following plots, unless stated otherwise).