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. 2023 Mar 24;10:1074700. doi: 10.3389/fcvm.2023.1074700

Figure 2.

Figure 2

Cardiomyocyte-specific TLR4 deletion attenuates angiotensin II-induced hypertension. (A) Mouse genotyping PCR using genomic DNA extracted from tail snips confirms that cardiomyocyte-specific TLR4 deletion (cTLR4KO) mice contain only the cTLR4KO band (300 bp, samples 4–7), as wild-type (WT) mice do not exhibit this band (samples 1–3.9). ntc, no template control; M, molecular weight marker. (B) Representative western blot image and quantification data indicate a significant reduction in cardiac TLR4 expression in cTLR4KO mice compared to WT mice (n = 4–6/group, unpaired, 2-tailed t-test, *p < 0.05 vs. WT + Saline). (C) Time course of blood pressure measurement using radiotelemetry showing angiotensin II (Ang II) infusion significantly increased the mean arterial pressure (MAP) in WT mice, however this effect in cTLR4KO mice was significantly attenuated (n = 10, repeated measures two-way ANOVA, *p < 0.05 vs. WT + Saline, †p < 0.05 vs. WT + Ang II). (D) MAP at day 14 of Ang II-infusion (n = 10, two-way ANOVA, *p < 0.05 vs. WT + Saline, †p < 0.05 vs. WT + Ang II).