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. 2023 Apr 6;14:1912. doi: 10.1038/s41467-023-37614-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Left, the single-cell expression of CD70 in EBV+ and EBV- NPC cells (tumor sample n = 36, two-sided Wilcoxon signed-rank test); Right, the quantified CD70+ cell fractions in EBV+ and EBV- NPC43 cells (n = 3, two-sided unpaired t test). b The chromatin accessibility measured by ATAC-seq at the CD70 promoter in EBV+, EBV- NPC cells, and NPE cells. c Top 15 enriched TFs with the highest transcriptional activity in EBV+ NPC cells. d The Pearson correlation (two-sided) between each TF and CD70 in GSE102349 (n = 112) and GSE68799 (n = 42) cohorts. e The spatial co-localization of NFKB2 and CD70 expression in down-sampled spatial spots (n = 100, two-sided Pearson correlation analysis with the 95% confidence band). f The Pearson correlation (two-sided) between NFKB2 and CD70 expression in EBV-transformed lymphocytes (n = 107, with the 95% confidence band). g Expression of NFKB2 across major cell lineages in the NPC scRNA-seq cohort (n = 50, two-sided Wilcoxon signed-rank test). h Relative firefly luminescence normalized by renilla luminescence in each experimental group (n = 3, two-sided unpaired t test). i, j The change of CD70 + fraction in shNC, shNFKB2, vehicle-treated, and SN-52-treated C666 cells (n = 3, two-sided unpaired t test). k The 18s-normalized mRNA fold change of CD70 in shNC, shNFKB2, vehicle-treated, and SN-52-treated C666 cells (n = 3, two-sided unpaired t test). l T-cell cytotoxicity measured in the shNC and shNFKB2 PBMC co-culture systems (n = 5, two-sided unpaired t test). m T-cell cytotoxicity measured in PBMCs co-cultured with vehicle and SN-52-treated C666 cells (n = 5, two-sided unpaired t test). n The schematic illustration of the molecular mechanism of the EBV-NFKB2-CD70-CD27 signaling axis and feedback loop between NPC cells, CD4+ naïve T cells/Tregs, and CD8+ T cells in the NPC microenvironment. The n number represents n biologically independent spots/samples/experiments in each group. The data are presented as the mean ± SD (bar plots), median ± IQR (whiskers = 1.5 × IQR, box & whiskers plots), and KDE (violin plots).