Table 1.
Effect of collagen type I on macrophage protein expression.
| Gene name | Protein name | Fold change | p-value |
|---|---|---|---|
| Serpinf1 | Pigment epithelium-derived factor | 35.82 | 4.74E−03 |
| Fam118b | Protein FAM118B | 13.21 | 2.45E−02 |
| Gbp4 | Guanylate-binding protein 4 | 10.34 | 6.90E−03 |
| Ndrg1 | Protein NDRG1 | 6.22 | 4.74E−03 |
| Syap1 | Synapse-associated protein 1 | 6.02 | 2.45E−02 |
| Nudt9 | ADP-ribose pyrophosphatase, mitochondrial | 5.95 | 1.35E−02 |
| Golt1b | Vesicle transport protein GOT1B | 5.27 | 2.05E−03 |
| Sptlc1 | Serine palmitoyltransferase 1 | 5.18 | 3.23E−03 |
| Arfgap3 | ADP-ribosylation factor GTPase-activating protein 3 | 5.02 | 6.90E−03 |
| Thop1 | Thimet oligopeptidase | 4.93 | 9.70E−03 |
| Slain2 | SLAIN motif-containing protein 2 | 4.49 | 6.90E−03 |
| Ethe1 | Persulfide dioxygenase ETHE1, mitochondrial | 4.14 | 1.35E−02 |
| Mrps14 | 28S ribosomal protein S14, mitochondrial | 3.95 | 1.82E−02 |
| Trmt2a | tRNA (uracil-5-)-methyltransferase homolog A | 3.37 | 3.23E−03 |
| Eml2 | Echinoderm microtubule-associated protein-like 2 | 3.19 | 6.90E−03 |
| Alox5 | Polyunsaturated fatty acid 5-lipoxygenase | 3.19 | 4.74E−03 |
| Ftl1 | Ferritin light chain 1 | 3.11 | 1.82E−02 |
| Ctnnd1 | Catenin delta-1 | 2.52 | 1.82E−02 |
| Nelfa | Negative elongation factor A | 2.45 | 9.70E−03 |
| Pfkl | ATP-dependent 6-phosphofructokinase, liver type | 2.38 | 9.70E−03 |
| Esyt2 | Extended synaptotagmin-2 | 2.35 | 6.90E−03 |
| Grhpr | Glyoxylate reductase/hydroxypyruvate reductase | 2.34 | 3.23E−03 |
| Acad8 | Isobutyryl-CoA dehydrogenase, mitochondrial | 2.26 | 2.45E−02 |
| Tm9sf3 | Transmembrane 9 superfamily member 3 | 2.18 | 2.45E−02 |
| Fam162a | Protein FAM162A | 2.12 | 1.82E−02 |
| Agpat4 | 1-acyl-sn-glycerol-3-phosphate acyltransferase delta | 2.11 | 2.45E−02 |
| Armc10 | Armadillo repeat-containing protein 10 | 2.10 | 6.90E−03 |
| F13a1 | Coagulation factor XIII A chain | 2.06 | 1.82E−02 |
| Ero1a | ERO1-like protein alpha | 2.04 | 9.70E−03 |
| Lrp1 | Low-density lipoprotein receptor-related protein 1 | 2.01 | 4.31E−04 |
| Casp9 | Caspase-9 | 0.46 | 1.35E−02 |
| Racgap1 | Rac GTPase-activating protein 1 | 0.38 | 1.29E−03 |
| Nabp2 | SOSS complex subunit B1 | 0.30 | 1.82E−02 |
30 proteins were expressed at levels more than twofold higher by macrophages cultured on collagen type I-coated plastic (fibrous and globular combined, n = 6 each), compared to macrophages cultured on uncoated plastic (n = 6). Three proteins were expressed at levels more than twofold lower (fold change < 0.5). p < 0.025 was considered significant.