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. 2023 Mar 24;14:1142240. doi: 10.3389/fimmu.2023.1142240

Figure 3.

Figure 3

Genetic deletion of NK-1R alleviated UUO-induced renal fibrosis, inflammation, and apoptosis. (A, B) RT-qPCR (A) and Western blotting (B) displayed the reduced mRNA (A) and protein (B) levels of Collagen I, α-SMA, MCP-1, and TNF-α in renal cortical tissues of NK-1R knockout (NK-1R-/-) UUO mice, compared with NK-1R wildtype (NK-1R+/+) UUO mice. (C) Immunochemistry staining of NK-1R and F4/80, PAS, Masson’s trichrome, and TUNEL analysis showed that the deletion of NK-1R attenuated UUO-induced renal fibrosis, infiltration of F4/80-positive inflammatory cells, and apoptosis. For (A–C), mouse kidneys were excised on day 14 after UUO. Analysis of TUNEL-positive cells was counted by fluorescence microscopy in ten randomly selected high-power fields per kidney section. Scale bar, 50 µm. Data are shown as mean ± SEM from groups of six mice. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.