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. 2023 Apr 7;8:52. doi: 10.1038/s41541-023-00653-7

Fig. 5. The glycosylated nanoparticle 145S confers long-lived protective antibody responses.

Fig. 5

a C57BL/6J mice were immunized with 10 µg or 0.5 µg immunogen 145S adjuvanted with LMQ or with LMQ alone at day 0 and 28. The serum IgG response against the repeat (NANP) and the junction (NPDP) was measured at different timepoints. One representative out of two independent experiments with 10 mice per group is shown until day 50. Day 51 until day 141 shows data from one experiment with 5 mice per group. b Anti-repeat (NANP) or junction (NPDP) IgG response at day 50 and day 141 after the first immunization. Dots represent individual mice. Day 50: Pooled data of three independent experiments with 5 mice per group are shown. Day 141: Data of one experiment with 5 mice per group is shown. Symbols indicate independent experiments. c Capacity of pooled sera (diluted 1:800) collected 50 days or 141 days after first immunization to inhibit the hepatocyte traversal activity of Pf sporozoites in vitro. Pooled data of three independent traversal assay experiments are shown. Symbols indicate independent immunization experiments. d, e Capacity of immunization with immunogen 145S at two different doses to protect mice after PbPfCSP(mCherry)-infected mosquito bite challenge (3 bites per mouse) from parasitemia. Data show the percentage of blood stage parasite-free mice over time. d Day of challenge 51: Pooled data of two independent experiments with 5 mice per group are shown. e Day of challenge 142: Data from one experiment with 5 mice per group. Arithmetic mean (a–c) and SEM (a) are indicated. Statistically-significant differences were calculated by two-tailed Mann–Whitney test (*P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001). Statistically non-significant differences are not indicated.