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. 2023 Mar 23;14:1103392. doi: 10.3389/fimmu.2023.1103392

Table 2.

True KO signaling pathways identified by SPIA from GSE22873.

Order Pathway ID Pathway Name Status pSize DEGs (%) pGFdr
Pathways identified from GSE22873_M (KO Myd88 vs. WT)
1 mmu04620 Toll-like receptor signaling pathway Inhibited 87 36 (41.38) 1.03E-33
2 mmu05161 Hepatitis B Inhibited 149 36 (24.16) 1.20E-24
3 mmu05140 Leishmaniasis Inhibited 66 23 (34.85) 9.51E-20
4 mmu05152 Tuberculosis Inhibited 158 32 (20.25) 2.20E-19
5 mmu04621 NOD-like receptor signaling pathway Inhibited 148 30 (20.27) 6.91E-18
6 mmu05162 Measles Inhibited 131 28 (21.37) 6.91E-18
7 mmu05142 Chagas disease (American trypanosomiasis) Inhibited 99 23 (23.23) 4.43E-15
8 mmu05169 Epstein-Barr virus infection Inhibited 185 29 (15.68) 1.16E-14
9 mmu05164 Influenza A Inhibited 143 26 (18.18) 1.40E-14
10 mmu05145 Toxoplasmosis Inhibited 105 22 (20.95) 2.62E-14
11 mmu05170 Human immunodeficiency virus 1 infection Inhibited 194 28 (14.43) 2.16E-13
12 mmu05135 Yersinia infection Inhibited 116 22 (18.97) 7.84E-13
13 mmu05133 Pertussis Inhibited 67 17 (25.37) 2.36E-12
14 mmu04064 NF-kappa B signaling pathway Inhibited 92 18 (19.57) 1.94E-10
15 mmu05168 Herpes simplex virus 1 infection Inhibited 340 31 (9.12) 3.64E-10
16 mmu05132 Salmonella infection Inhibited 193 24 (12.44) 5.73E-10
17 mmu05134 Legionellosis Inhibited 51 13 (25.49) 2.56E-09
18 mmu05235 PD-L1/PD-1 checkpoint pathway in cancer Inhibited 84 15 (17.86) 1.38E-08
19 mmu04010 MAPK signaling pathway Inhibited 281 25 (8.90) 3.65E-07
20 mmu05144 Malaria Inhibited 48 9 (18.75) 1.23E-05
21 mmu05143 African trypanosomiasis Inhibited 31 7 (22.58) 0.000126
Pathways identified from GSE22873_MA (KO Myd88_Ager vs. WT)
1 mmu05170 Human immunodeficiency virus 1 infection Inhibited 194 61 (31.44) 2.18E-50
2 mmu04620 Toll-like receptor signaling pathway Inhibited 87 44 (50.57) 1.24E-45
3 mmu05169 Epstein-Barr virus infection Activated 185 53 (28.65) 8.65E-41
4 mmu05142 Chagas disease (American trypanosomiasis) Inhibited 99 35 (35.35) 2.30E-29
5 mmu05162 Measles Inhibited 131 38 (29.01) 1.42E-28
6 mmu05161 Hepatitis B Inhibited 149 40 (26.85) 1.63E-28
7 mmu05135 Yersinia infection Inhibited 116 35 (30.17) 1.61E-26
8 mmu05235 PD-L1/PD-1 checkpoint pathway in cancer Inhibited 84 31 (36.91) 1.94E-26
9 mmu05168 Herpes simplex virus 1 infection Inhibited 340 50 (14.71) 6.55E-24
10 mmu04621 NOD-like receptor signaling pathway Activated 148 33 (22.30) 1.24E-20
11 mmu05140 Leishmaniasis Inhibited 66 23 (34.85) 3.92E-19
12 mmu05164 Influenza A Inhibited 143 31 (21.68) 7.33E-19
13 mmu05152 Tuberculosis Inhibited 158 31 (19.62) 1.74E-17
14 mmu05133 Pertussis Inhibited 67 21 (31.34) 2.00E-16
15 mmu05145 Toxoplasmosis Inhibited 105 23 (21.91) 1.60E-14
16 mmu04064 NF-kappa B signaling pathway Inhibited 92 22 (23.91) 5.09E-14
17 mmu05132 Salmonella infection Inhibited 193 26 (13.47) 5.18E-11
18 mmu04933 AGE-RAGE signaling pathway in diabetics Activated 98 18 (18.37) 1.58E-09
19 mmu05134 Legionellosis Inhibited 51 13 (25.49) 7.03E-09
20 mmu04010 MAPK signaling pathway Inhibited 281 22 (7.83) 6.66E-06
21 mmu05144 Malaria Inhibited 48 9 (18.75) 2.00E-05
22 mmu05143 African trypanosomiasis Inhibited 31 7 (22.58) 0.000149
23 mmu05010 Alzheimer disease Activated 311 13 (4.18) 0.158326
Pathways identified from GSE22873_A (KO Ager vs. WT)
1 mmu04933 AGE-RAGE signaling pathway in diabetics Activated 98 10 (10.20) 0.002314
2 mmu05150 Staphylococcus aureus infection Activated 76 3 (3.95) 0.476408
3 mmu05010 Alzheimer disease Activated 311 8 (2.57) 0.638874

True positive knockout (TPKO) signaling pathway (pGFdr < 0.001 or 0.005) and false negative knockout (FNKO) signaling pathway (pGFdr > 0.005) are distinguishable. Bold pathways are discussed in the main text.