Table 1.
Antibodies and reagents used.
| Reagent | Manufacturer | Article number | Dilution ratio | Positive control |
|---|---|---|---|---|
| Anhydrous ethanol | Sinopharm Group Chemical Reagent Co., Ltd | 100092683 | ||
| Xylene | Sinopharm Group Chemical Reagent Co., Ltd | 10023418 | ||
| Antibody dilution | Dako Denmark A/S | S2022 | ||
| EDTA antigen repair solution | Dako Denmark A/S | JYF-9062 | 1:50 | |
| PBS buffer | Fuzhou Maixin Biotechnology Development Co., Ltd | PBS-0060 | ||
| 3% hydrogen peroxide | Sinopharm Group Chemical Reagent Co., Ltd | 10011218 | ||
| DAB color development kit | Dako Denmark A/S | K5007 | ||
| Hematoxylin dye | Self-prepared reagent | |||
| Hydrochloric acid ethanol solution | Sinopharm Group Chemical Reagent Co., Ltd | |||
| Ammonia solution | Sinopharm Group Chemical Reagent Co., Ltd | 10002118 | ||
| Neutral gum | Sinopharm Group Chemical Reagent Co., Ltd | 10004160 | ||
| Nrf2 primary antibody | Wuhan Bode Biological Co., Ltd | PB9290 | 1:200 | Human breast carcinoma tissue |
| HO-1 primary antibody | Wuhan Bode Biological Co., Ltd | BA0605 | 1:200 | Human spleen tissue |
| NQO1 primary antibody | Wuhan Bode Biological Co., Ltd | BM4978 | 1:50 | Human breast adenocarcinoma tissue sections |
| BRAF V600E primary antibody | Beijing Zhongshan Jinqiao Biotechnology Co., Ltd | ZA-0668 | Working fluid | Human melanoma tissue |
| CK-19 primary antibody | Fuzhou Maixin Biotechnology Development Co., Ltd | Kit-0030 | Working fluid | with B-RAF V600E mutation |
| Gal-3 primary antibody | Fuzhou Maixin Biotechnology Development Co., Ltd | MAB-0572 | Working fluid | Human stomach adenocarcinoma tissue |
| Secondary antibody | DAKO REALTMEnVision | K5007 | Human colon cancer tissues | |
| Goat non-immune serum | Fuzhou Maixin Biotechnology Development Co., Ltd | SP KIT-B3 |