Figure 4. Immune Cell Subsets and T-Cell Receptor (TCR) Sequencing.

Peripheral blood samples were obtained at baseline and at first follow-up (3 months). A, For flow cytometry gating, parent populations are the CD3⁺ population for both CD4⁺ and CD8⁺, the CD4⁺ population for all markers on CD4⁺ cells including T-regulatory cells (Tregs), the CD8⁺ population for all markers on CD8⁺ cells, and the Treg population for the Treg CD73⁺ population. Asterisks indicate P < .05 for the W-score comparing the population between the 3-month follow-up timepoint and the baseline timepoint. CTLA-4 indicates cytotoxic T-lymphocyte associated protein 4; ICOS, inducible costimulator; LAG-3, lymphocyte activation gene 3; MDT, metastasis-directed therapy; PD-1, programmed cell death protein 1; TIGIT, T-cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domains; TIM3, T-cell immunoglobulin and mucin domain 3.