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. 2023 Apr 7;13:5707. doi: 10.1038/s41598-023-32745-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Pathologic analysis using Masson’s Trichrome-stained lung specimens. (A,B) The representative microphotographs of the lung in the normal mice (upper panel) and IP mouse models treated with MTX for 1 week (middle panel) and 2 weeks (bottom panel). (A) Con group or Con-IP group (B) Si group or Si-IP group. Scale bar 100 µm (A,B: left); 50 µm (A,B: right).