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. 2023 Apr 7;13:5707. doi: 10.1038/s41598-023-32745-8

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© The Author(s) 2023

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Immunofluorescence staining using α-SMA. The representative microphotographs of the lung in the normal mice (upper panel) and IP mouse models treated with MTX for 1 week (middle panel) and 2 weeks (bottom panel). Left panel: Con group or Con-IP group; right panel: Si group or Si-IP group. Scale bar: 20 µm.