Fig. 3.

CRISPR-associated transposon and recombination systems. a Cas transposases include both Cas proteins and transposase-associated components. Cargo DNA is identified by its left end (LE) and right end (RE) sequences and bound by transposase proteins (Tns). Guide RNA binding to Cas nuclease brings transposase to the specific site, and transposase integrates the DNA cargo into the target site. The target site is duplicated and flanks the integrated LE–cargo–RE sequence. Each Cas-transposase complex has a specific requirement for the spacer length of guide RNA and a unique position preference of integration site. b reassembly of guide RNA-programmed recCas9 at the target sites