Table 1. Evaluation of Antibody Combinations for Development of the pS129-α-Synuclein Electrochemiluminescence ELISAa.
| capture antibody | detection antibody | LoB (pg/mL) | LLoD (pg/mL) | LLoQ (pg/mL) | ULoQ (pg/mL) | intra-assay CV (%) | interassay CV (%) |
|---|---|---|---|---|---|---|---|
| biotin-EP1536Y (anti-pS129-α-syn) | anti-human α-syn-Sulfo tag (MSD) | 3.1 ± 0.6 | 6 ± 3 | 14 ± 10 | 66,167 | 3.9 | 7.2 |
| biotin-anti-human α-syn (MSD) | EP1536Y-Sulfo tag | 11 ± 3 | 15 ± 8 | 75 ± 13 | 33,088 | 4.6 | 10.5 |
| biotin-MJFR1 (anti-human α-syn) | EP1536Y-Sulfo tag | 11 ± 10 | 32 ± 10 | 97 ± 30 | 33,088 | 7.2 | 28.3 |
a
Mean ± SD are shown for LoB, LLoD, and LLoQ.