Table 1. Average Deuterium Incorporation Difference between Free and Complex States of Trastuzumab and Pertuzumab Measured at the Intact Antibody and Subunit Levela.
|
Average HDX protection in affimer complex
(ΔDa) |
|||
|---|---|---|---|
| Protein form (+glycan) | 60 s HDX | 300 s HDX | 600 s HDX |
| Trastuzumab Intact (G0F/G0F) | 17.00 (±3.06) | 17.77 (±6.16) | 16.90 (±8.91) |
| Trastuzumab F(ab′)2 | 12.47 (±1.32) | 16.67 (±6.60) | 14.37 (±5.70) |
| Trastuzumab Fc/2 + G0F | –0.67 (±0.33) | 1.33 (±1.36) | 0.97 (±1.29) |
| Pertuzumab Intact (G0F/G0F) | 13.77 (±2.99) | 21.53 (±3.23) | 20.83 (±0.44) |
| Pertuzumab F(ab′)2 | 17.73 (±1.53) | 18.10 (±0.44) | 18.50 (±0.50) |
| Pertuzumab Fc/2 + G0F | 1.03 (±0.43) | 0.97 (±0.26) | 1.63 (±0.37) |
a
Standard error of mean in brackets.