Table 3.
Primer sequences used for quantitative real-time polymerase chain reaction
|
Gene
|
Sequence (5’ to 3’)
|
|
| GAPDH | F: AGAAGGCTGGGGCTCATTTG | R: AGGGGCCATCCACAGTCTTC |
| DR4 | F: CCAGGCAGCATTGAAGTCAG | R: GTTTCCAGCATCACCAGGGT |
| DR5 | F: CCCTGGAGTGACATCGAATG | R: CAGCCACAATCAAGACTACGG |
| FADD | F: TGCGGGAGTCACTGAGAATC | R: GGAGGTAGATGCGTCTGAGTTC |
| Caspase-8 | F: GAGAAGCAGCAGCCTTGAAG | R: GACAGTATCCCCGAGGTTTG |
| Caspase-3 | F: CCCTCCTCAGCATCTTATCC | R: TGGTACAGTCAGAGCCAACCT |
| Cyto-C | F: CACAAGACTGGGCCAAATCTC | R: CCAGGGATGTACTTCTTGGGA |
| XIAP | F: CTATGCTCACCTAACCCCAAG | R: TTCTGACCAGGCACGATCAC |
| c-FLIP | F: GGAACCCTCACCTTGTTTCG | R: CTCCTTGCTTATCTTGCCTCG |
| Bcl-2 | F: CCTTCTTTGAGTTCGGTGGG | R: CGGTTCAGGTACTCAGTCATCC |
| Bax | F: CACCAGCTCTGAGCAGATCA | R: TGTTACTGTCCAGTTCGTCCC |
DR4: Death receptor 4; DR5: Death receptor 5; FADD: Fas-associated death domain protein; Cyto-C: Cytochrome C; XIAP: X-chromosome linked inhibitor-of-apoptosis protein; c-FLIP: Cellular Fas associated death domain-like interleukin-1β converting enzyme inhibitory protein; Bcl-2: B-cell lymphoma-2; Bax: Bcl-2-associated X protein.