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. 2023 Apr 7;4(4):101346. doi: 10.1016/j.xcrp.2023.101346

Figure 1.

Figure 1

ACE2 and heparin binding affinity to VOC spike trimer

(A) Binding affinities of VOC spikes to dimeric ACE2 measured by BLI; KD values were calculated with steady-state analysis.

(B) Degree of bound complex formation for VOC spikes to dimeric ACE2 as measured by ELISA. TMB (3,3′,5,5′-tetramethylbenzidine) was used as a chromogen for ELISA.

(C) Binding affinities of VOC spikes to HEP measured by BLI; KD values calculated with steady-state analysis.

(D) Degree of bound complex formation for VOC spikes to HEP as measured by ELISA. Three independent tests were performed (n ≥ 3), and standard deviation from mean value was represented as an error bar in the graph. p values < 0.05 (∗), 0.01 (∗∗), and 0.001 (∗∗∗) were determined using a one-way ANOVA with Tukey’s post hoc test.