Table 4.

TP53 mutations found from study subjects

Frequency of detection	Exon	Codon	Base substitution	Amino acid change	Mutation Type	Within DNA binding Domain	Hotspota
1	E05	387	CT	A129A	Heterozygous	Yes	Not detected
1	E05	413	CT	A138V	Heterozygous	Yes	Yes
1	E05	477	CT	A159A	Heterozygous	Yes	Yes
1	E06	572_574	delCTC	A189A	Homozygous	Yes	Yes
1	E06	566	CT	A189V	Heterozygous	Yes	No
1	E08	919	GA	A307T	Heterozygous	No	Not detected
1	E05	405	CT	C135C	Heterozygous	Yes	Yes
1	E05	404	GA	C135Y	Heterozygous	Yes	Yes
1	E05	423_427	delCCCTG	C140Cfs*6	Heterozygous	Yes	No
1	E05	423	CT	C141C	Heterozygous	Yes	Yes
1	E05	527	GT	C176F	Heterozygous	Yes	Yes
1	E05	442	GT	D148Y	Heterozygous	Yes	No
1	E06	624	CT	D208D	Heterozygous	Yes	Yes
1	E06	592	GA	E198K	Heterozygous	Yes	Yes
1	E06	610	GT	E204X	Heterozygous	Yes	Yes
1	E08	811	GT	E271X	Heterozygous	Yes	No
1	E08	813	GA	E271E	Heterozygous	Yes	Yes
1	E08	811	GA	E271K	Heterozygous	Yes	Yes
1	E08	854	AG	E285G	Homozygous	Yes	No
1	E08	856	GA	E286K	Heterozygous	Yes	No
1	E08	859	GT	E287X	Heterozygous	Yes	Yes
1	E08	880	GT	E294X	Heterozygous	No	Yes
1	E08	892	GA	E298K	Heterozygous	No	Yes
1	E05	461	GA	G154D	Heterozygous	Yes	Yes
1	E05	534	CT	H178H	Heterozygous	Yes	Yes
1	E05	536	AG	H179R	Heterozygous	Yes	Yes
1	E05	486	CT	I162I	Heterozygous	Yes	Yes
1	E05	485	TC	I162T	Heterozygous	Yes	No
1	E06	583	AT	I195F	Heterozygous	Yes	Yes
1	E08	915	GA	K305K	Homozygous	No	Yes
1	E08	866	TC	L289P	Homozygous	Yes	No
1	E08	865	CG	L289V	Heterozygous	Yes	No
1	E05	426	TC	P142P	Heterozygous	Yes	Yes
1	E05	452	CA	P151H	Heterozygous	Yes	Yes
2	E05	452	CT	P151L	Heterozygous	Yes	Yes
1	E05	457	CT	P153S	Heterozygous	Yes	No
1	E05	530	CT	P177L	Heterozygous	Yes	No
1	E06	667	CT	P223S	Heterozygous	Yes	Yes
1	E05	408	AG	Q136Q	Homozygous	Yes	Yes
1	E05	430	CT	Q144X	Homozygous	Yes	No
1	E05	493	CT	Q165X	Heterozygous	Yes	No
1	E05	523	CT	R175C	Heterozygous	Yes	Yes
1	E05	524	GA	R175H	Heterozygous	Yes	Yes
2	E06	586	CT	R196X	Heterozygous	Yes	Yes
1	E06	626	GA	R209K	Heterozygous	Yes	Yes
3	E06	637	CT	R213X	Heterozygous	Yes	No
1	E06	638	GT	R213L	Heterozygous	Yes	Yes
1	E08	801	GA	R267R	Heterozygous	Yes	Yes
1	E08	846	GA	R282R	Heterozygous	Yes	Yes
1	E08	844	CT	R282W	Homozygous	Yes	Yes
1	E08	869	GT	R290L	Heterozygous	No	Yes
2	E08	916	CT	R306X	Heterozygous	No	Yes
1	E05	419	CT	T140I	Heterozygous	Yes	No
1	E06	632	CT	T211I	Heterozygous	Yes	Yes
1	E08	919	delG	T304P	Heterozygous	No	No
1	E05	428	TC	V143A	Heterozygous	Yes	No
1	E05	517	GT	V173L	Heterozygous	Yes	Yes
1	E06	609	GA	V203V	Homozygous	Yes	Yes
1	E08	814	GA	V272M	Heterozygous	Yes	Yes
1	E05	489	CA	Y163X	Heterozygous	Yes	No
1	E05	489	CT	Y163Y	Heterozygous	Yes	Yes
1	E06	613	TC	Y205H	Heterozygous	Yes	Yes
1	E06	659	AG	Y220C	Heterozygous	Yes	Yes
1	E05	376-28_413	delCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTGCCCTCAACAAGATGTTTTGCCAACTGGC		Heterozygous	No	Not detected
1	E08	894_895	insAG		Heterozygous	No	Yes

This form made in accordance with the nomenclature rules of the Human Genome Variation Society (http://www.HGVS.org/varnomen). del, deletion; ins, insertion; fs, frame shift

^a According to the TP53 Database (R20, July 2019): https://tp53.isb-cgc.org